DNA

Part:BBa_K4397006

Designed by: Rashik Chand   Group: iGEM22_NYU_Abu_Dhabi   (2022-10-02)


Tau Aptamer 3146

Tau Aptamer: 3146

Target: Tau 441, Tau 381, Tau 352, and Tau 383

Binding Affinity: 13 nM, 116 nM, 84 nM, and 49 nM (SPR)


Usage and Biology

DNA aptamer 3146 is obtained from a 5 × 1012 DNA oligonucleotide library in only three rounds that can be performed within one day [1]. Target of 3146 aptamer is 2N4R-Tau, with selectivity for a monomer and with the Kd value of 13 nM (SPR) [2]. Aptamer 3146 bound tau isoforms in the following order: 2N4R (KD = 13 nM) > 0N4R (49 nM) > 0N3R (84 nM) > 1N3R (116 nM) [2].


[1]Storhoff, J. J., Elghanian, R., Mucic, R. C., Mirkin, C. A., & Letsinger, R. L. (1998). One-pot colorimetric differentiation of polynucleotides with single base imperfections using gold nanoparticle probes. Journal of the American Chemical Society, 120(9), 1959-1964.

[2] Murakami, K., Obata, Y., Sekikawa, A., Ueda, H., Izuo, N., Awano, T., ... & Irie, K. (2020). An RNA aptamer with potent affinity for a toxic dimer of amyloid β42 has potential utility for histochemical studies of Alzheimer's disease. Journal of Biological Chemistry, 295(15), 4870-4880.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None